Consensus statement from the second RdRp Summit: towards a unified framework for RNA virus biology

RNA-dependent RNA polymerase, or RdRp, remains the central molecular hallmark of RNA viruses. It serves as both a universal anchor for virus detection and a critical target for understanding the functional and evolutionary properties of RNA viruses. Since the inaugural RdRp summit in 2023, there have been significant advances in sequencing, structural prediction and artificial intelligence, all of which have accelerated the pace of RNA virus discovery and taxonomic annotation, revealing unprecedented levels of viral diversity, including novel phyla and unique genome architectures. The second RdRp summit, which was held in Lisbon in May 2025, gathered a group of research scientists from diverse subfields of virology to address emerging challenges in RNA virus biology. These challenges ranged from standardising annotation and data sharing to harnessing structure-guided phylogenetics and planetary-scale computational tools. Here, our consensus statement outlines key progress, current and future challenges and community-driven initiatives, including benchmarking, virus-host inference, and ongoing knowledge exchange efforts - all of which are designed to unify the field. By fostering an environment of sustained collaboration, our efforts aim to build a coherent framework for modern RNA virus biology and to accelerate the exploration of the hidden RNA virosphere.

Recommended citation: Lucaci A, Shaikh H, Chong LC, Tahzima R, Forgia M, Ben Mansour K, Sakaguchi S, Nakagawa S, Hou X, Demina T, Jayaraj Mallika FR, Kupczok A, Lytras S, Debat H, Charon J, Urzo M, Raco M, Kim S, Rivero Herrera R, Karapliafis D, Sirkinti L, Luebbert L, Nishimura L, Chikhi R, De Coninck L, CHARRIAT F, Soufir E, Gajdov V, Krannich T, Dudas G, Lood C, Rodríguez-Ramos J, Pecman A, Neri U, Werner A, Le M, Osundahunsi B, Petersen N, Maclot F, Gutierrez S, Paraskevopoulou S, Hillary L, Olendraitė I, 2025. "Consensus statement from the second RdRp Summit: towards a unified framework for RNA virus biology". Zenodo https://doi.org/10.5281/zenodo.17725152
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